NESA Biology Genetic Technologies

This order is incorrect because it suggests creating recombinant DNA before extracting the target DNA, and selecting organisms before the DNA is even introduced into the host.

This is the correct logical sequence: first choose the vector and host, extract the target DNA, ligate it into the vector to create recombinant DNA, introduce it into the host, and finally select the successful transformants.

This sequence is incorrect because it starts with introducing recombinant DNA into the host before the target DNA has been extracted or the recombinant molecule has been created.

This order is incorrect because it places the selection of organisms containing recombinant DNA before the recombinant DNA is actually created and introduced into the host.

DNA ligase is essential for recombinant DNA technology as it acts as molecular glue to join the sugar-phosphate backbones of the DNA fragment and the vector.

Plasmid vectors are required to act as vehicles that carry the foreign DNA into a host cell for replication and maintenance.

Restriction enzymes are necessary to cut both the vector and the source DNA at specific sequences, creating compatible ends for insertion.

Transcription factors are proteins that regulate gene expression (transcription) inside a cell, but they are not tools used to construct or assemble recombinant DNA molecules.