NESA Biology Cell Replication
15 sample questions with marking guides and sample answers · Avg. score: 73.1%
What is a key difference between spermatogenesis and oogenesis?
Spermatogenesis produces haploid cells, whereas oogenesis produces diploid cells.
Spermatogenesis produces four functional gametes, whereas oogenesis produces one.
Spermatogenesis occurs throughout life, whereas oogenesis only occurs after puberty.
Spermatogenesis begins with haploid cells, whereas oogenesis begins with diploid cells.
Reveal Answer
Spermatogenesis produces haploid cells, whereas oogenesis produces diploid cells.
Both spermatogenesis and oogenesis are forms of meiosis that result in the production of haploid gametes () from diploid germ cells ().
Spermatogenesis produces four functional gametes, whereas oogenesis produces one.
Spermatogenesis results in four equally sized, functional sperm cells, whereas oogenesis involves unequal cytokinesis, producing one large functional ovum and smaller, non-functional polar bodies.
Spermatogenesis occurs throughout life, whereas oogenesis only occurs after puberty.
Spermatogenesis begins at puberty and continues throughout life, whereas oogenesis begins during fetal development, arrests, and resumes at puberty.
Spermatogenesis begins with haploid cells, whereas oogenesis begins with diploid cells.
Both processes begin with diploid stem cells (spermatogonia and oogonia) that differentiate into diploid primary spermatocytes and oocytes before undergoing meiosis.
Explain the role of the enzymes helicase and DNA polymerase in the process of DNA replication.
Reveal Answer
Helicase unzips the DNA molecule by breaking the weak hydrogen bonds between the two complementary strands.
This creates a replication fork region so that bases are exposed.
DNA polymerase uses each original strand as a template to produce a copy of the DNA molecule, and adds complementary nucleotides to the exposed bases.
DNA polymerase also proofreads the newly synthesised strand.
| Descriptor | Marks |
|---|---|
States helicase’s role in unzipping DNA | 1 |
States helicase’s role in exposing bases | 1 |
States DNA polymerase’s role in adding complementary nucleotides to the exposed bases | 1 |
States another role of helicase, polymerase, or suitable feature of the process | 1 |
In the structure of DNA, adenine pairs with which other base?
uracil
guanine
cytosine
thymine
Reveal Answer
uracil
Uracil is found in RNA, where it pairs with adenine. In DNA, thymine replaces uracil.
guanine
Guanine pairs with cytosine via three hydrogen bonds, not with adenine.
cytosine
Cytosine pairs with guanine via three hydrogen bonds, not with adenine.
thymine
In the DNA double helix, adenine (A) always pairs with thymine (T) via two hydrogen bonds, consistent with Chargaff's rules.
Polymerase chain reaction (PCR) is a laboratory procedure used in DNA sequencing. PCR mimics cellular
DNA replication.
DNA repair.
transcription.
translation.
Reveal Answer
DNA replication.
Correct. PCR is an in vitro technique used to amplify specific DNA segments, directly mimicking the cellular process of DNA replication where a cell copies its genetic material.
DNA repair.
Incorrect. DNA repair involves identifying and fixing damaged DNA strands, whereas PCR is designed to synthesize new copies of a DNA template rather than fix errors.
transcription.
Incorrect. Transcription is the cellular process of synthesizing RNA from a DNA template, while PCR synthesizes new DNA strands.
translation.
Incorrect. Translation is the process of building proteins from an mRNA template at the ribosome, which is entirely different from the DNA amplification performed in PCR.
What is the role of DNA polymerase in DNA replication?
breaking the hydrogen bonds that hold the complementary bases of DNA together
adding complementary nucleotide bases to the exposed DNA strands
sealing the sequence of DNA into two continuous double strands
joining RNA primers to the lagging strand of DNA
Reveal Answer
breaking the hydrogen bonds that hold the complementary bases of DNA together
This describes the function of DNA helicase, which unwinds the double helix by breaking hydrogen bonds between base pairs.
adding complementary nucleotide bases to the exposed DNA strands
DNA polymerase is the enzyme responsible for synthesizing new DNA strands by adding nucleotides that are complementary to the template strand.
sealing the sequence of DNA into two continuous double strands
This describes the function of DNA ligase, which joins DNA fragments (such as Okazaki fragments) together to form a continuous strand.
joining RNA primers to the lagging strand of DNA
RNA primers are synthesized by the enzyme primase, not DNA polymerase, to initiate DNA synthesis.
If 28% of the bases in a DNA molecule are cytosine, what percentage will be adenine?
22
28
44
56
Reveal Answer
22
According to Chargaff's rules, cytosine pairs with guanine (28% + 28% = 56%), leaving 44% for adenine and thymine. Since adenine and thymine pair equally, adenine makes up half of 44%, which is 22%.
28
This is the percentage of guanine, which pairs equally with cytosine. Adenine pairs with thymine, so it will have a different percentage.
44
This represents the combined total percentage of both adenine and thymine (), not adenine alone.
56
This is the combined total percentage of cytosine and guanine () in the DNA molecule.
The haploid number of chromosomes in an orangutan is 24. How many chromosomes will be present in the sperm cell of an orangutan?
12
24
48
60
Reveal Answer
12
Incorrect. This is half of the haploid number, but gametes contain the full haploid set of chromosomes, not half of it.
24
Correct. Sperm cells are gametes, which are haploid cells, meaning they contain the exact haploid number of chromosomes ().
48
Incorrect. This is the diploid number (), which represents the chromosome count in somatic (body) cells, not reproductive sperm cells.
60
Incorrect. This is an arbitrary number that does not relate to the haploid () or diploid () chromosome counts of an orangutan.
Which combination of processes demonstrates spermatogenesis?
| Where process occurs | Outcome of process | |
|---|---|---|
| A | testes | one functional gamete with 2-3 polar bodies |
| B | ovaries | four functional haploid gametes |
| C | testes | four functional haploid gametes |
| D | ovaries | one functional gamete with 2-3 polar bodies |
Row A
Row B
Row C
Row D
Reveal Answer
Row A
While spermatogenesis occurs in the testes, the production of a single functional gamete with polar bodies is the outcome of oogenesis, not spermatogenesis.
Row B
Spermatogenesis occurs in the testes, not the ovaries; furthermore, the process occurring in the ovaries (oogenesis) typically results in only one functional gamete.
Row C
Spermatogenesis takes place in the testes and results in the production of four functional haploid gametes (sperm) from a single diploid precursor cell.
Row D
This option describes the process of oogenesis, which occurs in the ovaries and results in one functional egg cell and non-functional polar bodies.
Sheep have 54 chromosomes, while goats have 60 chromosomes. The hybrid offspring of a sheep-goat pairing is called a geep.
During fertilisation, an egg from the sheep is fertilised by a sperm from the goat, resulting in a geep.
Which of the following will be correct for the geep?
chromosomes
chromosomes
chromosomes
chromosomes
Reveal Answer
chromosomes
Incorrect. The geep receives 27 chromosomes from the sheep and 30 from the goat, making its total chromosome count 57, not a haploid number of 29.
chromosomes
Incorrect. The total chromosome count is determined by adding the haploid numbers of the parents (), not 52.
chromosomes
Correct. The sheep egg provides a haploid set of 27 chromosomes () and the goat sperm provides 30 chromosomes (), resulting in a diploid geep with chromosomes.
chromosomes
Incorrect. This is the sum of the parents' diploid numbers (). Fertilization involves the fusion of haploid gametes, not diploid cells.
The role of helicase in DNA replication is to
initiate the process by binding to recognition sites along the template strand.
add complementary bases to the template strand.
unwind and separate DNA strands.
join DNA strands together.
Reveal Answer
initiate the process by binding to recognition sites along the template strand.
This describes the function of initiator proteins or origin recognition complexes, which bind to specific DNA sequences to recruit helicase to the origin of replication.
add complementary bases to the template strand.
Adding complementary nucleotides to synthesize the new DNA strand is the function of DNA polymerase, not helicase.
unwind and separate DNA strands.
Helicase breaks the hydrogen bonds between nitrogenous bases, effectively unzipping the double helix to create the replication fork.
join DNA strands together.
Joining DNA fragments (such as Okazaki fragments) by sealing nicks in the sugar-phosphate backbone is the role of DNA ligase.
The following steps occur in DNA replication:
I. DNA uncoils
II. DNA is proofread
III. DNA is synthesised
IV. DNA unzips
Which of the following lists these steps in the order in which they occur?
II, I, IV, III
IV, II, III, I
III, II, I, IV
I, IV, III, II
Reveal Answer
II, I, IV, III
Proofreading (II) occurs after the new DNA strand is synthesized, not at the beginning of the replication process.
IV, II, III, I
DNA must uncoil (I) before it can unzip (IV), and proofreading (II) occurs after synthesis (III), not before.
III, II, I, IV
DNA synthesis (III) requires the DNA to be uncoiled (I) and unzipped (IV) first in order to expose the template strands.
I, IV, III, II
DNA replication begins with the double helix uncoiling (I) and unzipping (IV) to expose the template strands, followed by the synthesis of the new strand (III), which is then proofread for errors (II).
Which of the following statements about meiosis is correct?
Both crossing over and independent assortment of chromosomes occur during meiosis I.
Both crossing over and independent assortment of chromosomes occur during meiosis II.
Crossing over occurs during meiosis I and independent assortment of chromosomes occurs during meiosis II.
Crossing over occurs during meiosis II and independent assortment of chromosomes occurs during meiosis I.
Reveal Answer
Both crossing over and independent assortment of chromosomes occur during meiosis I.
This is correct because crossing over happens during prophase I when homologous chromosomes pair up, and independent assortment occurs during metaphase I when these pairs align randomly. Both processes generate genetic diversity during meiosis I.
Both crossing over and independent assortment of chromosomes occur during meiosis II.
This is incorrect because neither process occurs during meiosis II. Meiosis II involves the separation of sister chromatids, whereas crossing over and independent assortment involve homologous chromosomes during meiosis I.
Crossing over occurs during meiosis I and independent assortment of chromosomes occurs during meiosis II.
This is incorrect because independent assortment occurs during metaphase I of meiosis I, not meiosis II. During meiosis II, sister chromatids separate, but the random assortment of homologous pairs has already taken place.
Crossing over occurs during meiosis II and independent assortment of chromosomes occurs during meiosis I.
This is incorrect because crossing over occurs during prophase I of meiosis I, not meiosis II. By the time meiosis II begins, homologous chromosomes have already been separated into different cells.
A key difference between binary fission and mitosis is that
binary fission is a type of nuclear division, but mitosis is not.
homologous chromosomes pair during mitosis, but not in binary fission.
DNA replication occurs during binary fission, but not during mitosis.
binary fission produces two daughter cells, but mitosis does not.
Reveal Answer
binary fission is a type of nuclear division, but mitosis is not.
Incorrect. Mitosis is specifically the division of the nucleus in eukaryotes, while binary fission occurs in prokaryotes which do not have a nucleus.
homologous chromosomes pair during mitosis, but not in binary fission.
Incorrect. The pairing of homologous chromosomes is a key feature of meiosis, not mitosis or binary fission.
DNA replication occurs during binary fission, but not during mitosis.
Correct. In eukaryotes, DNA replication happens before mitosis during the S phase of the cell cycle, whereas in prokaryotes, DNA replication occurs simultaneously with the process of binary fission.
binary fission produces two daughter cells, but mitosis does not.
Incorrect. Both binary fission and mitosis are forms of cell division that result in the production of two genetically identical daughter cells.
A DNA molecule includes
thymine and deoxyribose sugar.
uracil and deoxyribose sugar.
guanine and ribose sugar.
adenine and ribose sugar.
Reveal Answer
thymine and deoxyribose sugar.
This is correct because DNA (deoxyribonucleic acid) is composed of nucleotides that contain a deoxyribose sugar and one of four nitrogenous bases, including thymine.
uracil and deoxyribose sugar.
While DNA does contain a deoxyribose sugar, uracil is a nitrogenous base found exclusively in RNA, replacing the thymine found in DNA.
guanine and ribose sugar.
Although guanine is a nitrogenous base found in DNA, ribose is the sugar backbone of RNA. DNA contains deoxyribose sugar instead.
adenine and ribose sugar.
Adenine is a base found in DNA, but ribose is the sugar found in RNA. The sugar in a DNA molecule is deoxyribose.
Which of the following are broken as a normal part of DNA replication?
hydrogen bonds between complementary bases
nitrogen bonds between complementary bases
phosphodiester bonds between adjacent nucleotides
phosphate bonds between adjacent nucleotides
Reveal Answer
hydrogen bonds between complementary bases
This is correct. During DNA replication, the enzyme helicase unzips the double helix by breaking the weak hydrogen bonds between complementary nitrogenous bases, allowing each strand to act as a template.
nitrogen bonds between complementary bases
This is incorrect. Complementary bases are held together by hydrogen bonds, not nitrogen bonds, which do not exist in this context.
phosphodiester bonds between adjacent nucleotides
This is incorrect. Phosphodiester bonds form the strong sugar-phosphate backbone of the DNA strand, which must remain intact while the two strands are separated.
phosphate bonds between adjacent nucleotides
This is incorrect. The phosphate-based bonds connecting adjacent nucleotides make up the structural backbone of the DNA strand and are not broken to separate the strands.